Composite

Part:BBa_K4800054:Design

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-09)


pTrc99a-davB-davA-GabT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5122
    Illegal XbaI site found at 6489
    Illegal PstI site found at 6501
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5122
    Illegal PstI site found at 6501
    Illegal NotI site found at 2808
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5122
    Illegal BglII site found at 3897
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5122
    Illegal XbaI site found at 6489
    Illegal PstI site found at 6501
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5122
    Illegal XbaI site found at 6489
    Illegal PstI site found at 6501
    Illegal NgoMIV site found at 4057
    Illegal AgeI site found at 3464
    Illegal AgeI site found at 3713
    Illegal AgeI site found at 5535
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6685
    Illegal BsaI site found at 7753
    Illegal SapI.rc site found at 767


Design Notes

Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.


Source

None

References